1.) Choose “Explore Data” in banner at top of each page
2.) Select to review data from one of three tiers
Cohort-Level Data
Select disease from the drop-down box to:
- Compare somatic mutations and gene expression for cohorts of our PDOX models and matched patient tumors, stratified by disease and subtype
- Review TSNE plots for methylation subtype classifications
- Interrogate chemical sensitivity profiling for single point, dose response, and combination datasets
Sample-Level Data
- Search and filter a table with the full list of models
- Review sample details for each model, including nucleotide sequencing performed, clinical features, and material (PDOX or cell line) available for request
- Compare histology for PDOX and matched patient tumor
- View somatic mutations, copy number variations, and FISH, as applicable
- Confirm disease and subtype classification based on DNA methylation
Gene-Level Data
- Search PDOX and matched patient tumors for somatic mutations
- Generate custom heatmaps for single or multiple genes
- View specific single nucleotide variations, and insertions/deletions for PDOX and matched patient tumor
- Link to sample details for each model
Review our FAQs for more information.
1.) Choose “Explore Data” in banner at top of each page
2.) Select Sample-Level Data to search and filter models
3.) Select models of interest and choose request samples in the drop-down menu
- Please note that only PDOX or cell line models are available for request. Patient material is not available.
- Review the protocol for orthotopic implant and cell culture before requesting.
4.) Complete the request form and submit
- Following submission, you will receive an email indicating we have received your request. Our governance committee reviews all requests. Once approved, we will ask that you sign a Materials Transfer Agreement (MTA) prior to sample shipment.
- PDOX sample availability may vary, as orthotopic brain tumors grow at different rates (some > 300 days) and yield low cell count. We will attempt to honor all requests, but there may be a delay in shipment due to availability. We will contact you if there is a delay or suggest alternate models, depending on your intended use.
- Please limit your requests to three PDOX. Larger requests will be reviewed on a case-by-case basis.
Cryo-preserved vials of PDOX or SJ cell lines are available for request here. We do not provide patient material, including tissue or nucleotides.
Orthotopic brain tumor PDOX models require significant resources to expand and bank. We ask that you be conscientious and limit requests to three PDOX lines. Larger requests will be reviewed on a case-by-case basis.
Commercial entities may contact [email protected] for details.
Yes. If you indicate that you do not have experience with orthotopic xenografts, we will provide a test sample. Please also consult our protocols for orthotopic implant and cell culture prior to request. We are happy to answer questions related to technique and protocols. Please contact [email protected].
We do not charge for the materials. You will be responsible for shipping charges. Cost for shipping will vary depending on domestic/international shipment and courier.
Commercial entities may contact St. Jude Cloud Support for details.
No, PBTP actively tracks sample distribution so we can provide updated sample information should this become available. All interested investigators should submit a request.
We do not provide additional curated data outside of that provided on PBTP. For raw data, please visit St.Jude Cloud.
Enter the question in the comment section of the request form when you initially submit it, or send an email to [email protected].